ENDOTOXEMIA AND BACTERIAL MICROFLORA IN SMALL BOWEL OBSTRUCTION
ABDULLAH İĞCİ1, MEHMET EMİN GÜÇLÜ3, ALİ AĞAÇFİDAN2, BETİGÜL ÖNGEL2, MESUT PARLAK1, UĞURSEL GÜÇLÜ3, 4, KAYHAN GÜNAY1,
1İstanbul Üniversitesi Tıp Fakültesi, Genel Cerrahi A.B.D.; İSTANBUL
2İstanbul Üniversitesi Tıp Fakültesi, Genel Cerrahi A.B.D.; İSTANBUL
3Haseki Hastanesi, II Cerrahi Kliniği, İSTANBUL
4SSK Okmeydanı Hastanesi IV. Cerrahi Kliniği, İSTANBUL
The aim of this experimental study was to investigate luminal bacterial colonization and assess the most likely substance (endogenous endotoxin) responsible for the pathophysiologic alterations in small bowel obstruction. Ten Wistar Albino rats with small bowel obstruction, 10 sham-operated and 10 control animals were investigated for 72 hours. Qualitative and quantitative bacterial changes in the bowel lumen were evaluated at sacrifice. Endotoxine levels were determined in portal and central venous blood by using LAL (Limulus Amebocyte Lysate) test. In the rats with small bowel obstruction, 103-104 fold increased microflora with a predominance of E.Coli bacteria was observed in the terminal ileum (p<0.05). in each of the 10 rats an endotoxin release into the both portal and central venous circulation could be detected in the legation group (p<0.05), whereas a detectable endotoxin level was measured only in portal blood of 1 animal in sham legation, and 1 in control group (p>0.05).