Protective effects of St. John’s wort in the hepatic ischemia/reperfusion injury in rats
1General Surgery, Haydarpaşa Numune Training and Research Hospital, İstanbul, Turkey
2Department of Pharmacology, Marmara University School of Pharmacy, İstanbul, Turkey
3Department of Biophysics, Near East University School of Medicine, Nicosia, Turkish Republic of Northern Cyprus
4Department of Biochemistry, Marmara University Vocational School of Health Related Professions, İstanbul, Turkey
5Department of Histology-embryology, Marmara University School of Medicine, İstanbul, Turkey
6Department of Medical Biology, Marmara University School of Medicine, İstanbul, Turkey
7Department of Pharmacology, Near East University School of Dentistry, Nicosia, Turkish Republic of Northern Cyprus
Keywords: Apoptosis, inflammatory, ischemia/reperfusion, St. John’s wort
Objectives: The purpose of this study was to investigate possible protective effects of St. John’s wort in the hepatic ischemia/reperfusion injury.
Material and Methods: The hepatic artery, portal vein, and bile duct were all clamped for 45 minutes to induce ischemia in rats, and after that reperfusion for 1 hour. SJW was administrated orally, once a day for 3 days before ischemia/reperfusion. The aspartate aminotransferase, alanine aminotransferase, tumor necrosis factor, and interleukin levels were measured in the serum samples. Luminol chemiluminescence, lucigenin luminol chemiluminescence levels; myeloperoxidase. The sodium–potassium ATPase (Na+/K+ ATPase) activity was determined in the liver tissue, and caspase-3 and caspase-9 activity with the bcl-2/bax ratio were measured by the western blot analysis.
Results: The St. John’s wort administration recovered the aspartate aminotransferase, alanine aminotransferase, tumor necrosis factor, and IL-1β levels serum parameters meaningfully, while ischemia/reperfusion caused an increase in luminol chemiluminescence, lucigenin luminol chemiluminescence, myeloperoxidase, caspase-3, and caspase-9 activity and led to a decrease in the B-cell lymphoma-2/bcl-2-associated X protein (bcl-2/bax) ratio and the Na+/K+ ATPase activity.
Conclusion: The obtained results indicate protective effects of St. John’s wort on the ischemia/reperfusion injury through various mechanisms, and we are able to suggest that St. John’s wort can clinically create a new therapeutic principle.
Cite this paper as: Atalay S, Soylu B, Aykaç A, Velioğlu Öğünç A, Çetinel Ş, Özkan N, et al. Protetctive effects of St. John’s wort in the hepatic ischemia/reperfusion injury in rats. Turk J Surg 2018; 10.5152/turkjsurg.2018.3952.
This study was presented at the “Congress NameI FCC World Lab”, “22-26 June 2014”, “İstanbul, Turkey ”.
Ethics committee approval was received for this study from the ethics committee of Marmara University Experimental Animal Research Centre (18.11.2013/95.2013)
Not required in this study.
Concept - S.A., A.Ö.Ş., B.S.; Design - A.Ö.Ş., S.S., B.S.; Supervision - A.Ö.Ş., A.A, N.Ö.; Resource - Ş.Ç., A.V.Ö., A.A., Ö.Ş.; Materials - B.S., A.Ö.Ş., Ş.Ç., C.E.; Data Collection and/or Processing - A.A., B.S., C.E.; Analysis and/or Interpretation - A.Ö.Ş., S.A., B.S.; Literature Search - A.Ö.Ş., B.S., S.A.; Writing Manuscript - A.Ö.Ş., B.S., A.A.; Critical Reviews - A.Ö.Ş., B.S., S.A., B.S.
The authors have no conflicts of interest to declare.
The authors declared that this study has received no financial support.