BACTERIAL TRANSLOCATION IN OBSTRUCTIVE JAUNDICE

Abstract

In this experimental study, bacterial translocation was investigated in rats which have been occured obstructive jaundice via different periods. This study has been practised in completely sterilized conditions. Animals have been divided into 7 groups. Group I (n: 10) was called as normal group. Laparotomy has been applied for group II (n:10), group IV(n:10) and VI (n:10). Obstructive jaundice has been performed for 3 days in group III (n:12), for 7 days in group V (n:12) and for 21 days in group VII (n:12). After that bacterial translocation was investigated in systemic circulation and mesenteric lymph nodes. While bacterial translocation could not be detected in systemic circulation of normal group and laparotomy group, it was detected in mesenteric lymph nodes of them in 10% and 11% respectively. On the other hand, in the group that has been occured obstructive jaundice, bacterial translocation was detected in systemic circulation and mesenteric lymph nodes 17% and 42% in group III, 36% and 56% in group V, 56% and 73% in group VII respectively. Cecal population of jaundiced animals has been found to be increased 10 to 200 times especially for gr/-) aerob bacterias. Mucosal injury that is characterised by submucosal edema, inflamatory cellular infiltration and epithelial desquamation has been found in ileal specimens. All results have been studied statistically. Cecal bacterial population, severity of the histopathological findings of ileum, bacterial translocation through sistemic circulation and mesenteric lymph nodes have been found to be increased in accordance with the duration of obstruction.