THE EFFECT OF GRANULOCYTE COLONY STIMULATING FACTOR ON THE IMMUNE PARAMETERS IN EXPERIMENTAL OBSTRUCTIVE JAUNDICE

Abstract

Obstructive jaundice is an important clinical problem. Jaundice dependent complications are renal insufficiency, cardiovascular sequels, coagulation defects, gastrointestinal bleeding, delayed wound healing, secondary biliary cirrhosis and sepsis. In our experimental study we performed four groups that consist of 28 rats. First group consisted of sham rats. 2nd group consisted of sham and GM-CSF applied rats. 3 rd and 4th groups consisted of rats that had obstructive jaundice. In 4th group we applied 5 microgr/kg GM-CSF subcutaneously to the rats for 7 days. We measured the levels of neutrophis, lymphocytes, leukocytes, IFN-a CD32, CD34, CD64 in all groups.

In jaundice group neutrophils, lymphocytes and leukocytes counts are found significantly lower compared to other groups (p<0.05). IFN-a and CD levels are found lower in jaundiced group compared to other groups. In jaundice with GM-CSF applied group neutrophils, lymphocytes leukocytes and IFN-alpa levels are found higher CD32 levels are found higher in GM-CSF applied groups. The difference were found significant.

We concluded that GM-CSF is an effective factor to prevent serious and high mortality septic complications of jaundice and to restore defected macrophage-neutrophils functions and it seems to us GM-CSF can be placed therapeutic protocois in the obstructive jaundice after clinical studies.