ANTIOXYDANT EFFECTS OF HYPERTONIC SALINE RESUSCITATION OF HEMORRHAGIC SHOCK IN RATS

Abstract

The aim of this study was to investigate the effect of hypertonic saline solution (HTS) on the tissue injury caused by oxygen free radicals in rats. The abdominal aorta was catheterized and animals were bled via catheter until their mean arterial pressure came down to 35mmHg indicating the hemorrhagic shock established. After 60 minutes of shock, the animals were divided into two groups, each of which includes ten rats. First group resuscitated with HTS (7.5% NaCl, 4 ml/kg) plus shed blood and second group with twice the shed blood volume of lactated Ringer's (RL) plus shed blood. Arterial pressure was recorded before and during shock and in post-resuscitation period. Blood samples were obtained to determine plasma malondialdehyde levels. There was no significant difference between two groups with regard to arterial blood pressure. Post-resuscitation plasma malondialdehyde levels were lower in first group (HTS animals) compared with second group (RL animals) (p<0.05). Inconclusion, the results of this study showed that the resuscitation of hemorrhagic shock with HTS in rats decreased free radicals-induced tissue injury.