Evaluation of Echinococcus DNA by polymerase chain reaction (PCR) in cystic Echinococcosis of the liver
Mehmet Tolga Kırış1
, Sefa Ergün1, Ozan Akıncı1, Sevgi Ergin2, Mehmet Velidedeoğlu1, Bekir Sami Kocazeybek2, Ertuğrul Göksoy1
1Department of General Surgery, İstanbul University Cerrahpaşa Faculty of Medicine, İstanbul, Türkiye
2Department of Medical Microbiology, İstanbul University Cerrahpaşa Faculty of Medicine, İstanbul, Türkiye
Abstract
Objective: The aim of this study was to determine the DNA and genotypes of Echinococcus granulosus in liver cyst hydatids isolated in humans.
Material and Methods: This study was conducted prospectively at the Department of General Surgery of the Cerrahpaşa School of Medicine, University of İstanbul-Cerrahpaşa, between January 2015 and June 2016 in 30 patients who were operated on for cystic Echinococcosis. E. granulosus DNA was analyzed using the Polymerase Chain Reaction (PCR) method in the cyst samples (protoscolex and/or germinative membrane) obtained during the operation, and genotype was determined in the PCR positive samples by sequence analysis. At the same time, indirect hemagglutination (IHA) was used to test for the presence of antibodies in the patients’ blood.
Results: E. granulosus DNA was found in 29 out of 30 cystic Echinococcosis of the liver samples. All of the 29 cystic Echinococcosis samples were found to be G1 (sheep) species. Also, IHA was positive in 22 patients and negative in eight patients.
Conclusion: In the present study, G1 species was the most commonly seen liver cystic Echinococcosis species. We suggest that a vaccine, which could be developed against prevalent regional genotypes, would be efficacious in the prevention of the disease with a cause of mortality and morbidity.
Keywords: Liver cystic Echinococcosis, Echinococcus granulosus, genotype, DNA, PZR, Western-Blot
Cite this article as: Kırış MT, Ergün S, Akıncı O, Ergin S, Velidedeoğlu M, Kocazeybek BS, et al. Evaluation of Echinococcus DNA by polymerase chain reaction (PCR) in cystic Echinococcosis of the liver. Turk J Surg 2022; 38 (2): 196-201.
This study was approved by Cerrahpaşa Faculty of Medicine Research Ethics Committee (Decision no: 442684, Date: 07.12.2016).
Externally peer-reviewed.
Concept - M.T.K., S.E., E.G.; Design - M.T.K., S.E., B.S.K., E.G.; Supervision - S.E., O.A., E.G., M.V.; Materials - M.T.K., S.E., B.S.K., M.V.; Data Collection and/or Processing - M.T.K., S.E., O.A., B.S.K.; Analysis and/or Interpretation - S.E., O.A., S.E., E.G.; Literature Search - S.E., O.A., S.E.; Writing Manuscript - S.E., O.A.; Critical Reviews -S.E., S.E., E.G.
The authors have no conflicts of interest to declare.
The authors declared that this study has received no financial support.