Protective effects of spironolactone against hepatic ischemia/reperfusion injury in rats
Süleyman Atalay1, Belkıs Soylu2, Aslı Aykaç3, Ayliz Velioğlu Öğünç4, Şule Çetinel5, Naziye Özkan5, Can Erzik6, Ahmet Özer Şehirli7
1Department of General Surgery, Haydarpasa Numune Training and Research Hospital, İstanbul, Turkey
2Department of Pharmacology, Marmara University School of Pharmacy, İstanbul, Turkey
3Department of Biophysics, Near East University School of Medicine, Nicosia, Turkish Republic of Northern Cyprus
4Department of Biochemistry, Marmara University Vocational School of Health Related Professions, İstanbul, Turkey
5Department of Histology-Embryology, Marmara University School of Medicine, İstanbul, Turkey
6Department of Medical Biology, Marmara University School of Medicine, İstanbul, Turkey
7Department of Pharmacology, Near East University School of Dentistry, Nicosia, Turkish Republic of Northern Cyprus
Abstract
Objective: In the present study, it was aimed to study the antioxidant effects of spironolactone (SPL) to determine its possible protective effects in hepatic ischemia reperfusion injury.
Material and Methods: Hepatic artery, portal vein, and bile duct of Wistar albino rats were clamped for 45 minutes under anesthesia to form an ischemia period. Then reperfusion was allowed and the rats were decapitated 60 minutes later. SPL (20 mg/kg, p.o.) or SF was orally administered for 30 minutes before ischemia. Rats in the control arm underwent sham surgery and were administered isotonic saline. Liver function was studied by measuring aspartate aminotransferase (AST), alanine aminotransferase (ALT), tumor necrosis factor-alpha (TNF-α), and interleukin 1beta (IL-1β) levels. Malondialdehyde (MDA), glutathione (GSH), luminol, and lucigenin levels, myeloperoxidase (MPO) and Na+-K+-ATPase enzyme activities were analyzed to study tissue injury under light microscope.
Results: While IR increased AST, ALT, TNF-α, and IL-1β levels and MDA, luminol, and lusigenin levels and MPO activities, it caused a decrease in GSH levels and Na+K+-ATPase activity. Spironolactone administration significantly improved these values.
Conclusion: Protective effects of SPL against ischemia/reperfusion injury via various mechanisms suggest that this agent may become a novel treatment agent in clinical practice.
Cite this article as: Atalay S, Soylu B, Aykaç A, Velioğlu Öğünç A, Çetinel Ş, Özkan N, Erzik C, Şehirli AÖ. Protective effects of spironolactone against hepatic ischemia/reperfusion injury in rats. Turk J Surg 2019; 35 (4): 285-292.
This experiment was performed after obtaining the approval of the Local Ethics Committee at Marmara University Laboratory Animals Research Center with the protocol code numbered 80.2012.mar, dated 08.11.2012.
Written informed consent was obtained from patients who participated in this study.
Externally peer-reviewed.
Concept - S.A., B.S., A.Ö.Ş.; Design - S.A., A.Ö.Ş.; Supervision - S.A., A.Ö.Ş.; Resource - S.A., A.A., A.V.Ö., S.Ç., N.M., C.E.; Materials - S.A., B.S., A.Ö.Ş.; Data Collection and/or Processing - S.A., B.S., A.Ö.Ş.; Analysis and/or Interpretation - S.A., A.Ö.Ş.; Literature Search - S.A., B.S., A.Ö.Ş.; Writing Manuscript - All of authors; Critical Reviews - S.A., A.Ö.Ş.
The authors have no conflicts of interest to declare.
The authors declared that this study has received no financial support.