THE EFFECTS OF PROPHYLACTIC ANTIBIOTICS ON THE BACTERIAL TRANSLOCATION DUE TO PRINGLE MANEUVER

Abstract

This study was undertaken to investigate the effectiveness of prophylactic antibiotics (Cefotaxime and Ceftazidime) on the bacterial translocation related to PM in rats. Bacterial translocation may occur in humans and is associated with an increased incidence of septic morbidity. It has been consistently demonstrated in a number of experimental models,including burns,trauma,shock, and surgery. It has been previously determined that the PM causes bacterial translocation. The prophylactic antibiotics may reduce this translocation. Thirty male Sprague-Dawley rats weighed in at 250-300 gr were divided into 3 groups each were containing l0 rats. After being anaesthetized by intramuscularly injection of Ketamine, midline laparotomy was performed to all rats under sterile conditions. In group 1 (Control), the hepatoduodenal ligament was clamped for 40 min. In group 2 (Cefotaxime),following intramuscularly application of Cefotaxime (30 mg/kg) 30 minutes before the surgical procedures, the PM was performed for 40 min. In group 3 (Ceftazidime), Ceftazidime (30 mg/kg) was applied instead of Cefotaxime before the PM. 1ml of blood sample was obtained from the portal vein for blood culture. The MLN biopsy and splenectomy were performed for culture. The positive bacterial cultures, obtained from MLN, spleen and blood, were determined most frequently in group 1. It was found that the positive cultures of group 1 (56.7%) were significantly higher than those of group 2 (6.7%) and group 3 (6.7%) (p=0.006,p=0006,p=0,001,respectively). Microorgonisms, obtained from MLN and spleen,were E.coli, C.pneumonia, Mikst, Candida and E.fecalis (respectively, 61.9%, 14.3%, 9.5%, 9.5%, 4.8%). In both cultures of MLN and spleen, the CFU of the group 1 was significantly higher than CFU of group 2 and 3(p=0.001, p=0.003). In our study, we determined that PM significantly increased bacterial translocation in MLNs, spleen and blood, and prophylactic application of Cefotaxime and Ceftazidime had preventive effect on this translocation.