Turkish Journal of Surgery

Turkish Journal of Surgery

ISSN: 2564-6850
e-ISSN: 2564-7032

 

Orhan Alimoğlu1, Bülent Kaya1, Mustafa Şahin1, Muammer Kiraz2, Ramazan Eryılmaz1, Uğur Çevikbaş3, Yıldız Yeğenoğlu2

1Vakıf Gureba Eğitim Hastanesi, I. Cerrahi Kliniği, İSTANBUL
2İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Mikrobiyoloji AD, İSTANBUL
3İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Patoloji

Abstract

Purpose: Candida Albicans is the most common fungal pathogen that cause infection. Septic complications and renal failure are the most common causes of death in patients with obstructive jaundice. We investigated the translocation of oral administration of Candida albicans in rats with obstructive jaundice.

Materials and Methods: Forty male wistar-albino rats were divided into 4 Groups. In Group I, laparatomi was performed and without any intervention the abdomen was closed with 3/0 prolene suture. In group II, laparatomy was done and abdomen was closed. On first postoperative day, candida inoculation was administered intragastrically. In group III, following laparotomy, common bile duct was ligated and cut than the abdomen was closed. In group IV, common bile duct was ligated and cut and then on 3rd postoperative day oral candida inoculation was administered. On 7th day, blood from vena cava inferior and tissue samples from liver, mesenteric lymph nodes, and cecum were collected for pathological, microbiological and biochemical investigations from all rats.

Results: Microbiological lymph node positivity was statistically higher in group IV (p:0.020). When compared with Group II, candida colonization in cecum was higher in Group IV. Lymph node and cecum positivity were statistically higher in Group II and IV than Groups I and III (p<0.001). Histopathological changes in liver were statistically higher in group IV than in Groups I and III (p<0.05). Histopathological changes in the lymph nodes were statistically higher in group IV when compared with other Groups (p<0.01 and p<0.001). Histopathological changes in the cecum were statistically higher in Group IV when compared with Groups I and III (p<0.001 and p<0.05). Also, histopathological changes in the cecum were statistically higher in Group II than Group I (P<0.01).

Conclusion: Obstructive jaundice results in increase of colonisation and translocation of orally administrated Candida albicans.

Keywords: Candida albicans, obstructive jaundice, translocation.