Akın Köşüm1, Ersin Borazan2, Göktürk Maralcan2, Alper Aytekin2

1Department of General Surgery, Adıyaman Gölbaşı Hospital, Adıyaman, Turkey
2Department of General Surgery, Gaziantep University Faculty of Medicine, Gaziantep, Turkey

Abstract

Objective: In this study, biochemical and histopathological changes in renal functions were investigated to evaluate the effects of experimentally induced intra-abdominal hypertension.

Material and Methods: Thirty male Wistar albino rats were used in this experimental study. Rats were divided into four groups. Group 1 (control group, n=6) were only anesthetized. After the anesthesia, a 20 G intracatheter was introduced intraperitoneally to Group 2 (sham group, n=8), Group 3 (n=8) and Group 4 (n=8). Intra-abdominal pressure application was not performed in Group 2. We applied 20 mmHg intra-peritoneal pressure to Group 3 and 30 mmHg to Group 4 for 3 hours. After withdrawing 3 mL intracardiac blood from all groups, the kidneys were removed for histopathological examination. Serum urea and creatinine levels were measured in all groups.

Results: In the biochemical examination, blood urea and creatinine levels were statistically different among all groups (p<0.05). Serum creatinine levels in group 3 and serum urea and creatinine levels in group 4 were significantly increased. In the histopathological examination, the kidneys in Group 1 and Group 2 were evaluated as normal. In Group 3 congested areas were detected in the glomeruli and interstitial regions. In addition to these findings seen in Group 3 dilatations were detected in the pelvicaliceal structures and proximal ureters in Group 4.

Conclusion: When intra-abdominal pressure is increased, the levels of serum urea and creatinine are increased, because the kidneys were damaged. Foci of hemorrhage in interstitial area, dilatations in the proximal ureter, renal pelvis and lymphatics were the pathologic findings seen in the kidneys.

Keywords: Kidney, intra-abdominal hypertension, creatinine, histopathological changes, urea


 

Ethics Committee Approval

Ethics committee approval was received for this study from the ethics committee of Gaziantep University School of Medicine (30.06.2008, 06-2008/124).

Peer Review

Externally peer-reviewed.

Author Contributions

Concept - G.M., A.K.; Design - G.M., E.B., A.K.,; Supervision - G.M., E.B.; Funding - A.K., A.A.; Materials - A.K., A.A.; Data Collection and/or Processing - A.K., A.A.; Analysis and/or Interpretation - G.M., E.B., A.K.; Literature Review - A.K., A.A.; Writer - A.K., E.B., G.M.; Critical Review - G.M., E.B.

Conflict of Interest

No conflict of interest was declared by the authors.

Financial Disclosure

The authors declared that this study has received no financial support.