Xenotransplantation of human cryopreserved parathyroid tissue isolated from parathyroid adenomas to normocalcemic rabbits
Erhan Ayşan1, Yiğit Düzköylü2, İsmail Can3, Nur Büyükpınarbaşılı4
1Department of General Surgery, Bezmialem Vakıf University School of Medicine, İstanbul, Turkey
2Department of General Surgery, İstanbul Training and Research Hospital, İstanbul, Turkey
3Institute of Experimental Medicine, İstanbul University, İstanbul, Turkey
4Department of Pathology, Bezmialem Vakıf University School of Medicine, İstanbul, Turkey
Keywords: Hypoparathyroidism, xenotransplantation, parathyroid adenoma
Objective: Parathyroid allotransplantation is a new method for the treatment of permanent hypoparathyrodism. Adenoma cells are not used for transplantation because of the potential for functional or histopathologic transformation. In this study, we transplanted human adenomatous parathyroid cells to rabbits.
Material and Methods: Parathyroid adenoma tissue taken from a male patient was cryopreserved and transplanted into seven New Zealand white rabbits (mean weight, 3700±220 g; mean age, 4.5 months) under immunosuppression. The levels of parathormone, calcium and phosphorus were measured before and after transplantation, and the parathyroid cells were observed histopathologically.
Results: Mean parathyroid hormone level was 0.5 pg/dL before transplantation and 6.6 pg/dL after transplantation (p<0.05). Preoperative mean calciumlevel was 14.1 mg/dL, and mean phosporus level was 3.5 mg/dL before transplantation while these values were 14.4 mg/dL and 3.3mg/dL, respectively, after transplantation (p>0.05). Morphologic transformation was not observed in parathyroid cells after transplantation.
Conclusion: In short-term observation, adenomatous parathyroid cells can function without malignant transformation. In the future, the preliminary methodology in this study may serve as a safe alternative for allotransplantation into patients with permanent hypoparathyroidism.
Cite this paper as: Ayşan E, Düzköylü Y, Can İ, Büyükpınarbaşılı N. Xenotransplantation of human cryopreserved parathyroid tissue isolated from parathyroid adenomas to normocalcemic rabbits. Turk J Surg 2017; 33(2): 91-95
Ethics committee approval was received for this study from Bezmialem Vakıf University Animal Experiments Local Ethics Committee (Number 2013/99).
Not required in this study.
Concept - E.A.,Y.D.; Design - E.A., İ.C.; Supervision - E.A.,Y.D.; Resource - İ.C., N.P.; Materials - İ.C., N.P., E.A.; Data Collection and/or Processing - Y.D., N.P.; Analysis and/or Interpretation - E.A., İ.A.; Literature Search - Y.D., E.A.; Writing Manuscript - E.A., Y.D.; Critical Reviews - E.A.,Y.D.
No conflict of interest was declared by the authors.
The authors declared that this study has received no financial support.