Turkish Journal of Surgery

Turkish Journal of Surgery

ISSN: 2564-6850
e-ISSN: 2564-7032


Kutay Sağlam1, Etem Alhan1, Serdar Türkyılmaz1, Birgül Kural Vanizor2, Cengiz Erçin3

1Department of General Surgery, Karadeniz Technical University School of Medicine, Trabzon, Turkey
2Department of Medical Biochemistry, Karadeniz Technical University School of Medicine, Trabzon, Turkey
3Department of Pathology, Kocaeli University School of Medicine, Kocaeli, Turkey

Keywords: Hydrogen sulphide, pancreatitis, rats


Objective: The aim of this study was to investigate the dose-dependent anti-inflammatory effectof the Hydrogen sulfidedonor sodiumhydrosulphide on acute necrotizing pancreatitis in rats.
Material and Methods: A total of 42 male Sprague-Dawley rats were divided into 4 groups: sham+saline (group 1), sham+NaHS (group 2), acute necrotizing pancreatitis+saline (group 3), and acute necrotizing pancreatitis+NaHS (group 4). Acute pancreatitis was induced in rats in groups 3 and 4 with the infusion of glycodeoxycholic acidinto the biliopancreatic canal and infusion of cerulein parenterally. In group 4, 10 mg/kg NaHS was administered intraperitoneally after cerulein infusion.Tests for liver and kidney function, interleukin-6, lactate dehydrogenase in bronchoalveolar lavage, and malonyaldehyde and myeloperoxidase activities in pancreas and lung tissue were performed, and histopathologic examination of pancreas was conducted.
Results: In groups 3, a significant increase in amylase, alanine aminotransferase, urea, interleukine-6, lungmalondialdehydeand myeloperoxidase activities, pancreas myeloperoxidase activity, edema, and necrosis in pancreas tissue and a significant decrease in serum calcium levels were detected (p<0.05). In group 4, addition of NaHS resulted in a significant decrease in lactate dehydrogenase level in bronchoalveolar lavage, amount of urea, lung myeloperoxidase activity, and pancreatic edema (p<0.05).
Conclusion: Although not in pancreatic necrosis, hydrogen sulphide has an anti-inflammatory effect especially in the inflammatory process in lung and edema in pancreasin acute necrotizing pancreatitis at particular doses. With further studies evaluating the anti-inflammatory effects of hydrogen sulphide, we believe it can be used in the treatment of edematous acute pancreatitis and the related complications in lungs.

Cite this paper as: Sağlam K, Alhan E, Türkyılmaz S, Kural Vanizor B, Erçin C. The anti-inflammatory effect of hydrogen sulphide on acute necrotizing pancreatitis in rats. Turk J Surg 2017; 33: 158-163.

Ethics Committee Approval

Ethics committee approval was received for this study from the ethics committee of Karadeniz Technical University (05.05.2011-2011/14).

Peer Review

Externally peer-reviewed.

Author Contributions

Concept - K.S., E.A., S.T.; Design - K.S., E.A., S.T.; Analysis and/or Interpretation - B.K.V., C.E.; Literature Search - K.S.; Writing Manuscript - K.S., E.A.; Critical Reviews - K.S., E.A., S.T.

Conflict of Interest

No conflict of interest was declared by the authors.

Financial Disclosure

The authors declared that this study has received no financial support.